With the arrival of the lithography industry in Iran during the Qajar period, numerous printing houses were established in different cities. The first and most active stone printing house of Mashhad started operating in Astan Quds Razavi complex in 1294 AH. This printing house published 28 books in fifty years and finally in 1344 AD due to various factors, its activity ended. The purpose of the current research is to answer the question based on the descriptive-analytical method, what passive and accelerating factors have been effective on the activity process of Astan Quds Razavi stone printing house? The findings based on the comparative approach and the analysis of the points mentioned in the books left from this printing house indicate that passive factors include the attitude and successive changes of the trustees, political, military, and social tensions (domestic and foreign), conflicting interests of tradition and modernity. And the accelerating factors include the positive attitude of some trustees, scholars and other men, vows, donations and endowment of books, the presence of neighbors and pilgrims with a scientific-religious approach, and the activity of booksellers.