Atiq Courtyard is one of the oldest courtyards in this collection and Its construction was completed and built in the Safavid and Qajar periods. This courtyard has many artistic and architectural values, many of which have not yet been explained. On the other hand, in recent years, with the Appearance of neuroscience and its connection with art and architecture, insights based on human neurophysiology have been formed in art and architecture. Neuroesthetics is one of these insights which is a scientific field in the research on the perception of beauty. Consequently, the adaptation of the physical and spatial indices of this courtyard with the Neuroesthetics components can reveal the aesthetic values of this courtyard more. The purpose of this research is to identify and adapt the physical-spatial indicators of the Atiq courtyard and adapt it to the Neuroesthetics components. This research is of a qualitative type, in the first step, Neuroesthetics has been studied with a descriptive analytical method. Then, with the field Research and logical reasoning method, the physical-spatial indicators of this courtyard would be identified, and in the final step, the adaptation of the Neuroesthetics components with the physical-spatial Parameters of this courtyard would be done. The results of the research show that there are many Neuroesthetics components such as contrast, grouping, golden ratio and special proportions, connection with open space, height variation, natural and plant decorations in this courtyard, which can be the factors that stimulate the nervous system of visitors and induce the sense of beauty.